# N and O isotope effects of nitrate reductases # PI: Julie Granger (UConn) # Version: 12 Aug 2015 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 20 06182014 methyl_viologen cell_suspension p._denitrificans yes anaerobic 4 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 20.0 879.3 26.7 0.0 21.8 0.1 20.1 747.9 31.7 0.1 26.5 0.2 20.1 572.7 39.6 0.0 34.0 0.2 20.1 397.5 50.5 0.1 44.5 0.2 20.1 237.6 64.1 0.5 57.8 0.4 20.1 96.4 75.7 0.7 69.9 0.6 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 21 06182014 hydroquinone cell_suspension p._denitrificans yes anaerobic 4 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 21.0 970.2 24.5 0.0 19.2 0.3 21.1 945.0 25.2 0.0 19.9 0.6 21.2 844.2 27.7 0.0 21.4 1.6 21.3 869.4 28.3 0.0 22.8 0.5 21.4 811.4 29.4 0.1 23.7 0.8 21.5 781.8 30.2 0.0 24.7 0.9 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 22 06182014 hydroquinone cell_suspension p._denitrificans yes anaerobic 4 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 22.0 961.4 25.3 0.0 20.4 0.1 22.1 854.1 28.3 0.1 23.4 0.1 22.2 753.4 31.7 0.0 26.8 0.1 22.3 638.4 36.5 0.2 31.5 0.1 22.4 537.6 41.4 0.0 36.2 0.1 22.5 440.2 46.4 0.2 41.4 0.0 22.6 330.7 51.8 0.3 47.3 0.3 22.7 213.5 65.6 0.2 59.6 0.3 ========================= assay date reductant enzyme_form bacteria cells_lysed growth_conditions temp ------------------------- 23 06182014 hydroquinone cell_suspension p._denitrificans yes anaerobic 4 ========================= sample NO3_corr d15N d15N_sd d18O d18O_sd ------------------------- 23.0 935.1 25.7 0.1 20.3 0.0 23.1 816.9 29.2 0.1 24.1 0.1 23.2 648.2 34.4 0.0 29.1 0.1 23.3 579.3 38.1 0.0 33.4 0.1 23.4 442.4 46.4 0.0 40.6 0.0 23.5 325.2 54.2 0.4 48.2 0.0